NGHIÊN CỨU BỘ MÔN PHÂN TÍCH – KIỂM NGHIỆM

HƯỚNG NGHIÊN CỨU

Điều chế, chiết xuất, phân lập và thiết lập chất đối chiếu và cao đối chiếu sử dụng trong kiểm nghiệm thuốc.

Ứng dụng các phương pháp hóa lý (UV-Vis, FS, AAS, GC, LC, CE, MS) để xây dựng và thẩm định quy trình phân tích dược chất, chất chuyển hóa và chất đánh dấu sinh học trong dịch sinh học và quy trình phân tích các thuốc đa thành phần.

Tiêu chuẩn hóa dược phẩm, dược liệu, cao dược liệu định hướng tác dụng sinh học, dược mỹ phẩm và thực phẩm bảo vệ sức khỏe.

Nghiên cứu phương pháp phân tích bộ chất chuyển hóa (metabolomics) ứng dụng trong nghiên cứu Dược – Y – Sinh.

Phân tích đa dư lượng thuốc bảo vệ thực vật trong gia vị và dược liệu.

Tổng hợp và thiết lập tạp chất đối chiếu NDSRI (Nitrosamine Drug Substance Related Impurities) sử dụng trong kiểm nghiệm dược phẩm.

KẾT QUẢ NGHIÊN CỨU

Các công bố quốc tế (cập nhật đến ngày 31/12/2023)

• Hien Thi Thu Nguyen, Tho Van Duong, Dung Thanh Phan; Development and validation of HPLC method for simultaneous determination of amlodipine besilate, telmisartan and hydrochlorothiazide in combined tablets; MedPharmRes 2023, 7(4);  https://doi.org/10.32895/UMP.MPR.7.4.8.
• Chung Duong Dinh, Yen Nguyen Thi Ngoc, Dung Thanh Phan; UPLC-MS/MS Method for simultaneous determination of metformin and glimepiride in human plasma: A green approach to environment; Rasayan J. Chem 2022, 15(2), 1525-1533.

• Nai Ngoc Chuong, Nguyen Ho Nhat Nguyen, Hung Viet Tran, Tuan Duc Nguyen; Development and validation of a LC–MS/MS assay for tenofovir and tenofovir alafenamide in human plasma: Application in a bioequivalence study; MedPharmRes 2023, 7(3), 32-41.
• Tran Viet Hung, Ha Minh Hien, Pham Quoc Chinh, Pham Thi Thu Ha, Nguyen Duc Nhu, Nguyen Duc Tuan; Development and validation of a GC-MS method for determination of amphetamine-type stimulants and ketamine in human hair; Pharmaceutical Sciences Asia 2023, 50(1), 576-585.
• T. Nguyen Nguyen Le, Nai Ngoc Chuong, Tuan Duc Nguyen; A One-Step Sample Processing Method in Combination with HPLC-MS/MS for the Simultaneous Quantification of Atorvastatin, Ezetimibe and Three Metabolites including o-Hydroxyl Atorvastatin, p-Hydroxyl Atorvastatin, and Ezetimibe-Glucuronide in Human Plasma; Separations 2023, 10(7), 409);  https://doi.org/10.3390/separations10070409. 
• Phuc Van Hoang, Thy Dang Uyen Phan, Phuong Vu Quynh Duong, Anh Thi Van Tran, Khoi Tan Nguyen, Nam Van Ho Phan; Crucial Role of Surfactants in Improving the Extraction Efficiency of β‑Amyrin from Dischidia major Using Ultrasound-Assisted Extraction Coupled with Gas Bubble Flotation; Langmuir 2022, 38, 9285−9293.
• Thua Phong Lam, Dac Nhan Nguyen, Tan Thanh Mai, Thanh Dao Tran, Minh Tri Le, Phuong Nguyen Hoai Huynh, Duc Tuan Nguyen, Viet Hung Tran, Dieu Thuong Thi Trinh, Phuong Truong, Cam Van T. Vo, Khac Minh Thai; Exploration of chalcones as 3 chymotrypsin like protease (3CLpro) inhibitors of SARS CoV 2 using computational approaches; Structural Chemistry 2022. https://doi.org/10.1007/s11224-022-02000-3.
• Nai Ngoc Chuong, Thai Long Tran, Thanh Thi Ta, Son Van Pham, Hung Viet Tran, Tuan Duc Nguyen; Development and validation of simultaneous assay of simvastatin, beta-hydroxy simvastatin as metabolite in human plasma using liquid chromatography-tandem mass spectrometry; MedPharmRes 2022, 6(2), 9-17.
• Ha Thi Ngoc Tram, Do Nguyen Phuong Nam, Nguyen Thi Ngoc Dung, Nguyen The Han, Vo Thi Bach Hue, Phan Van Ho Nam. Acetylcholinesterase inhibitory activity of fatty acids isolated from brown seaweed Sargassum mcclurei Setchell. Journal of Medicinal Materials 2022, 27 (1), 19-24.
• Nguyen Thi Linh Tuyen, Le Quan Nghiem, Nguyen Duc Tuan, Phuoc Huu Le. Development of a Scalable Process of Film-Coated bi-Layer Tablet Containing Sustained-Release Metoprolol Succinate and Immediate-Release Amlodipine Besylate. Pharmaceutics 2021;13,1797. https://doi.org/10.3390/pharmaceutics13111797.
• Ngoc Nha Thao Nguyen, Duy Toan Pham, Duc Tuan Nguyen, Thi Thu Loan Trinh. Bilayer tablets with sustained?release metformin and immediate?release sitagliptin: preparation and in vitro/in vivo evaluation. Journal of Pharmaceutical Investigation 2021;51:579-586.
• Ngoc Chuong Nguyen, Dinh Vinh, Duc Tuan Nguyen, Huynh Van Thi Nguyen, Cong Luan Tran, Manh Hung Tran. Development of a Capillary Electrophoretic Method for the Determination of Huperzine A Concentration in Vietnamese Huperzia serrata. Natural Product Communications 2021;16(9):1-8. 
• Hoang Quoc Cuong, Nguyen Duc Hai, Hoang Thuy Linh, Nguyen Trung Hieu, Nguyen Hoang Anh, Tran Ton, Tran Cat Dong, Vu Thanh Thao, Do Thi Hong Tuoi, Nguyen Duc Tuan, Huynh Thi Kim Loan, Nguyen Thanh Long, Cao Minh Thang, Nguyen Thi Thanh Thao, Phan Trong Lan. The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era. BioMed Research International 2021. https://doi.org/10.1155/2021/5516344.
• Bao-Tan Nguyen, Thi Anh Tuyet Le, Xuan Lan Mai, Thi Ngoc Van Nguyen, Thanh Dung Phan, Jong-Seong Kang, Kyeong Ho Kim. Determination of enantiomeric impurity of tenofovir disoproxil fumarate on a cellulose tris(3,4-dichlorophenyl-carbamate) chiral stationary phase and the characterization of its related substances. Journal of separation science 2021;44(10):2029-2036.
• Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Sirine El Mousli, Emilie Secret, Jean-Michel Siaugue, Lac Thuy Huu Nguyen, Thanh Duc Mai. Electroosmotic flow modulation for improved electrokinetic preconcentration: Application to capillary electrophoresis of fluorescent magnetic nanoparticles. Analytica Chimica Acta 2021;1161:338466.
• Pham Van Son, Tran Long Thai, Chuong Ngoc Nai, Pham Thi Thanh Thao, Nguyen Duc Tuan, Tran Viet Hung, Nguyen Ngoc Vinh. Simultaneous determination of phyllanthin, hypophyllanthin, and niranthin in rabbit plasma by LC-MS/MS. International Journal of Pharmaceutical Research 2020;12(4):1974-1980.
• Chuong Ngoc Nai, Nguyen Van Trung, Tran Long Thai, Tran Viet Hung, Nguyen Duc Tuan. Simultaneous determination of amlodipine, losartan, and the losartan carboxylic acid active metabolite in human plasma by LC-MS/MS. International Journal of Pharmaceutical Research 2020;12(4):1981-1990.
• Hoang Thuy Linh, Trinh Xuan Tung, Trinh Huu Tung, Vo Quoc Bao, Le Thi Minh Hong, Pham Ngoc Thach, Nguyen Minh Ngoc, Luu Thanh Binh, Phan Trong Lan, Hoang Quoc Cuong, Nguyen Duc Hai, Nguyen Duy Long, Nguyen Thi Thu Phuong, Van Thi Thuy Linh, Pham Thi Mai Anh, Nguyen Thien Hai, Le Quan Nghiem, Nguyen Duc Tuan. Risk of microbiological contamination when preparing total parenteral nutrition for pediatric patients: A pilot study at a regional hospital in southern Vietnam. Systematic Reviews in Pharmacy 2020;11(2):1-5.
• Nguyen Ngoc Nha Thao, Nguyen Ngoc Hieu, Do Chau Minh Vinh Tho, Trinh Thi Thu Loan, Nguyen Duc Tuan. Development, validation, and application for simultaneous assay of metformin and sitagliptin in human plasma by liquid chromatography-tandem mass spectrometry. Systematic Reviews in Pharmacy 2020;11(2):6-13.
• Hoang Thuy Linh, Trinh Xuan Tung, Trinh Huu Tung, Vo Quoc Bao, Le Thi Minh Hong, Pham Ngoc Thach, Nguyen Minh Ngoc, Luu Thanh Binh, Phan Trong Lan, Hoang Quoc Cuong, Nguyen Duc Hai, Nguyen Duy Long, Nguyen Thi Thu Phuong, Van Thi Thuy Linh, Pham Thi Mai Anh, Nguyen Thien Hai, Le Quan Nghiem, Nguyen Duc Tuan. Parenteral nutrition preparation procedure: a hazard analysis and critical control points approach at a pediatric hospital in Vietnam. Systematic Reviews in Pharmacy 2020;11(1):159-164.
• Chuong Ngoc Nai, Nguyen Van Trung, Tran Long Thai, Nguyen Ngoc Vinh, Tran Viet Hung, Pham Van Son, Nguyen Duc Tuan. Development, validation, and application for simultaneous assay of amlodipine, atorvastatin, and ortho- and para-hydroxy atorvastatin as metabolites in human plasma by liquid chromatography tandem mass spectrometry. Systematic Reviews in Pharmacy 2020;11(1):165-173.
• Tho Chau Minh Vinh Do, Duy Quoc Nguyen, Tuan Duc Nguyen, Phuoc Huu Le. Development and validation of a LC-MS/MS method for determination of multi-class antibiotic residues in aquaculture and river waters, and photocatalytic degradation of antibiotics by TiO2 nanomaterials. Catalysts 2020;10(3):1-19.
• Phan Trong Lan, Hoang Quoc Cuong, Hoang Thuy Linh, Nguyen Trung Hieu, Nguyen Hoang Anh, Tran Ton, Tran Cat Dong, Vu Thanh Thao, Do Thi Hong Tuoi, Nguyen Duc Tuan, Huynh Thi Kim Loan, Nguyen Thanh Long, Cao Minh Thang, Nguyen Duc Hai, Nguyen Thi Thanh Thao. Development of standardized specimens with known concentrations for severe acute respiratory syndrome coronavirus 2 Realtime-RT-PCR testing validation. Bull World Health Organ. E-pub: 20 April 2020. doi: http://dx.doi.org/10.2471/BLT.20.259630.
• Thi Anh Tuyet Le, Thuy Vy Pham, Xuan Lan Mai, Chailin Song, Sunjung Woo, Cheolhee Jeong, Sungyoun Choi, Thanh Dung Phan, Kyeong Ho Kim. Enantiomeric purity test of R-(+)-alpha lipoic acid by HPLC using immobilized amylose-based chiral stationary phase. Analytical Science & Technology 2020;33(1):1-10.
• Do Chau Minh Vinh Tho, Huynh Phuong Thao, Nguyen Duc Tuan, Andreas Zemann. Quantitative analysis of S-Allylcysteine in black garlic via Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry. Systematic Reviews in Pharmacy 2019;10(1):161-166.
• Nguyen Thi Linh Tuyen, Le Quan Nghiem, Nguyen Duc Tuan. Simultaneous liquid chromatography-tandem mass spectrometry assay of amlodipine besylate and metoprolol succinate in human plasma: Development, validation, and application. Systematic Reviews in Pharmacy 2019;10(2):1-7.
• Trong Q. Luu, Phat T. Le, Khoa C. M. Le, An H. T. Phan, Kim Zitzmann, Kim T. Nguyen, Nam V. H. Phan, et al. Improving the quality of Vernonia amygdalina extract by ultrasound-assisted extraction coupled with gas bubble flotation. Journal of Food Process Engineering 2019;42(8):e13284.
• Nguyen Huy Viet Phan, Thi Kim Huong Nguyen, Thi Bach Hue Vo, Van Ho Nam Phan. Synthesis quercetin imprinted polymer by precipitation polymerization method, directed to apply as sorbent for selective solid-phase extraction. Pharmaceutical Sciences Asia 2019;46(2):108-119.
• Pol Van Nguyen, Trung Quang Vo, Thoai Dang Nguyen, Thuy Tran Chung Phan, Nam Van Ho Phan. Dengue fever in Southern of Vietnam: A survey of reported knowledge, attitudes, and practices. The Journal of The Pakistan Medical Association 2019;69(Suppl 2)(6):S118-S130.
• Thi-Ngoc-Dung Nguyen, Omid Feizbakhsh, Estelle Sfecci, Blandine Baratte, Claire Delehouzé, Andrien Garcia, et al. Kinase-based screening of marine natural extracts leads to the identification of a cytotoxic high molecular weight metabolite from the Mediterranean sponge Crambe tailliezi. Marine Drugs 2019;17(10):569.
• Nam V. H. Phan, Hermann F. Sussitz, Eva Ladenhauf, Dietmar Pum, Peter A. Lieberzeit. Combined Layer/Particle Approaches in Surface Molecular Imprinting of Proteins: Signal Enhancement and Competition. Sensors 2018;18(1):180; doi:10.3390/s18010180.
• Quoc-Ky Truong, Xuan-Lan Mai, Jae-Yong Lee, Jongsook Rhee, Dinh Vinh, Jongki Hong, et al. Simultaneous determination of 14 oral antihyperglycaemic drugs in human urine by liquid chromatography-tandem mass spectrometry. Archives of Pharmacal Research 2018;41:530-543.
• Augustine A. Ahmadu, Abdulkarim Agunu, Thi-Ngoc-Dung Nguyen, Blandine Baratte, Béatrice Foll-Rosselin, et al. Constituents of Acacia nilotica (L.) Delile with Novel Kinase Inhibitory Activity. Planta Medica International Open 2017;4:e108-e113.
• Kieu Xuan Hai, Phan Thanh Dung. Simultaneous determination of paracetamol, phenylephrine, chlorpheniramine and related compound 4-aminophenol in multi-components pharmaceuticals by high performance liquid chromatography. Mahidol Univ. J Pharm Sci 2016;43(1):37-44.
• Eva M. Ladenhauf, Dietmar Pum, Daniel S. Wastl, Jose Luis Toca-Herrera, Nam V. H. Phan, Peter A. Lieberzeit, Uwe B. Sleytr. S-layer based biomolecular imprinting. RSC Advances 2015;5: 83558-83564.
• Nam V. H. Phan, Hermann F. Sussitz, Peter A. Lieberzeit. Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP. Biosensors 2014;4(2):161-171.
• Tho Chau Minh Vinh Do, Tuan Duc Nguyen, Hung Tran, Hermann Stuppner, Markus Ganzera. Quantitative determination of phenolic compounds in Lotus (Nelumbo nucifera) leaves by capillary electrophoresis. Planta Medica 2013;78:1796-1799.
• Tho Chau Minh Vinh Do, Tuan Duc Nguyen, Hung Tran, Hermann Stuppner, Markus Ganzera. Analysis of alkaloids in Lotus (Nelumno nucifera Gaertn.) leaves by non-aqueous capillary electrophoresis using ultraviolet and mass spectrometric detection. Journal of Chromatography A 2013;1302:174-180.
• Hoang-Yen T. Nguyen, Bach-Hue T. Vo, Lac-Thuy H. Nguyen, Jose Bernad, Mohamad Alaeddine, Agnes Coste, Karine Reybier, Bernard Pipy, Françoise Nepveu. Extract of Crinum latifolium inhibit the cell viability of mouse lymph oma cell line EL4 and induce activation of anti-tumor activity of macrophages in vitro. Journal of Ethnopharmacology 2013;149(1):75-83.
• Thanh Dung Phan, Ngoc Hoan Nguyen, Dae Joong Kim, Yong Jae Lee, Seung Ho Choi, Kyeong Ho Kim. Determination of the L-enantiomer of Nateglinide in pharmaceutical formulation by micellar electrokinetic chromatography. Archives of Pharmacal Research 2010;33(12):2017-2024.
• Phan Thanh Dung, Tran Quoc Trung, Kyeong Ho Kim. Preparative Resolution of Etodolac enantiomers by Preferential Crystallization Method. Archives of Pharmacal Research 2009;32(10):1425-1431.
• Phan Thanh Dung, Mi Young Ko, Hyun Ju Choi, Kwan Seog Sin, Kyeong Ho Kim. Determination of Enantiomeric Impurity of Etodolac by Capillary Electrophoresis using (2-hydroxypropyl)- b-cyclodextrin. Archives of Pharmacal Research 2008;31(9):1218-1233.
• Tran Quoc Trung, Phan Thanh Dung, Nguyen Ngoc Hoan, Dae Joong Kim, Joo Huyn Lee, Kyeong Ho Kim. Chiral Separation of Fluvastatin by Capillary Electrophoresis. Archives of Pharmacal Research 2008;31(8):1066-1072.
• Wolfgang Gutleben, Karin Scherer, Nguyen Duc Tuan, Heribert Stoiber, Manfred P. Dierich, Andreas Zemann. Separation of 11 protease and reverse transcriptase inhibitors by capillary electrophoresis. Journal of Separation Science 2003;26:1198-1202.
• Nguyen Duc Tuan, Wolfgang Gutleben, Karin Scherer, Heribert Stoiber, Barbara Falkensammer, Manfred P. Dierich, Andreas Zemann. Simultaneous separation of fifteen approved protease and reverse transcriptase inhibitors for human immunodeficiency virus therapy by capillary electrophoresis. Electrophoresis 2003;24:662-670.
• Wolfgang Gutleben, Karin Scherer, Nguyen Duc Tuan, Heribert Stoiber, Manfred P. Dierich, Andreas Zemann. Simultaneous separation of 11 protease and reverse transcriptase inhibitors for human immunodeficiency virus therapy by co-electroosmotic capillary zone electrophoresis. Journal of Chromatography A 2002;982:153-161.
• Wolfgang Gutleben, Nguyen Duc Tuan, Heribert Stoiber, Manfred P. Dierich, Mario Sarcletti, Andreas Zemann. Capillary electrophoretic separation of protease inhibitors used in human immunodeficiency virus therapy. Journal of Chromatography A 2001;922:313-320.
• A. Zemann, D.T. Nguyen, G. Bonn. Fast separation of underivatized carbohydrates by co-electroosmotic capillary electrophoresis. Electrophoresis 1997;18:1142-1147.
• D.T. Nguyen, H. Lerch, A. Zemann, G. Bonn. Separation of derivatized carbohydrates by co-electroosmotic capillary electrophoresis. Chromatographia 1997;46(¾):113-121.

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